![]() ![]() See also Figure S1.ĭiurnal versus nocturnal mosquito species exhibit striking differences in their light-evoked attraction/avoidance behavior. coluzzii female and male mosquitoes for (H) daytime, light violet background indicates illuminated area and (I) nighttime, light violet background indicates previously illuminated area. (H-I) Average attraction/avoidance behavioral preference to UV light-exposed versus shaded-environment by Ae. (D-G) Attraction/avoidance behavior to UV light, measured by % of preference in UV-exposed versus shaded-environment during Zeitgeber time (ZT) 6–12 for (D) female Ae. aegypti (light green n=61), and (C) female An. (B-C) Attraction/avoidance behavior to UV light, measured by % of preference in UV-exposed versus shaded environment throughout 12 hr: 12 hr UV light: dark for (B) female Ae. (A) Schematic of mosquito light-evoked attraction/avoidance preference behavioral assay setup. UV light-evoked attraction/avoidance responses in diurnal and nocturnal mosquitoes are specie- and sex-dependent. coluzzii) mosquitoes based on comparative circadian interest and because both are anthropophilic mosquitoes that are major vectors of many human diseases. ![]() aegypti) and nocturnal Anopheles coluzzii ( An. We chose to investigate diurnal Aedes aegypti ( Ae. Despite their large impact on health and ecology, little is known about the basis of diurnality/nocturnality and behavioral timing in mosquitoes. Circadian clocks are light entrained and altered light timing disrupts circadian behaviors. Numerous mosquito behaviors change with time-of-day, including flight activity, mating, oviposition, and biting. Some mosquito species are diurnal (i.e., Aedes aegypti) while others are nocturnal (i.e., Anopheles coluzzii). ĭifferent mosquito species have evolved distinct circadian timing of behaviors according to their temporal/ecological niches. Recent work in flies shows that CRY mediates a wide range of behavioral responses to blue and UV light, including circadian modulated attraction/avoidance. Mosquitoes and flies additionally express non-opsin photoreceptors including the blue and UV light sensitive CRYPTOCHROME (CRY). It has been long assumed that insect responses to ultraviolet (UV) light are mediated by external photoreceptors including opsins in the eyes. Insects display a wide range of short wavelength light modulated behaviors, including attraction/avoidance. night behavioral profiles that changes with daily light: dark cycles. However, light-based insect controls do not typically take into consideration the day vs. Toxic pesticides are environmentally harmful in contrast to relatively safe light-based insect control approaches. Mosquito-spread diseases may have contributed to the deaths of half of all the people who have ever lived. night-biting mosquitoes, but both classes of mosquitoes are circadian and light regulated, which may be applied towards species-specific control of harmful mosquitoes. In summary, attraction/avoidance mosquito behaviors show marked differences between day- vs. Molecular disruption of the circadian clock severely interferes with light-evoked attraction/avoidance behaviors in mosquitoes. Observed differences at the neural circuit and protein levels provide insight into the fundamental basis underlying diurnality versus nocturnality. The basis of diurnal versus nocturnal behaviors is driven by molecular clock timing, which cycle in anti-phase between day- versus night-biting mosquitoes. ![]() The circadian neural circuits of diurnal/day- and nocturnal/night-biting mosquitoes based on PERIOD (PER) and Pigment-Dispersing Factor (PDF) expression show similar but distinct circuit organizations between species. Males of both diurnal and nocturnal mosquito species show reduced UV light avoidance in anticipation of evening onset relative to females. Behavioral attraction/avoidance to light in both species change with time-of-day and show distinct sex and circadian neural circuit differences. In contrast, night-biting mosquitoes Anopheles coluzzii, specifically avoid ultraviolet (UV) and blue light during the day. Day-biting mosquitoes Aedes aegypti, particularly females, are attracted to light during the day regardless of spectra. night-biting female and male mosquitoes’ innate temporal attraction/avoidance behavioral responses to light and their regulation by circadian circuit and molecular mechanisms. night-biting mosquitoes occupy distinct time-of-day niches. ![]() Mosquitoes pose widespread threats to humans and other animals as disease vectors. ![]()
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